FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_cDNA3P_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_cDNA3P_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132843414
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13179670.9921206933149129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2500100.034.2535361
AGCAGTG2804700.030.415052
TCAACGC3001100.027.51464712
GTGGTAT3107050.027.3241256
ATCAACG3044200.027.31155411
CAACGCA3024100.027.29973213
AGTGGTA3164800.026.9042995
TATCAAC3136750.026.56325510
CGCAGAG3169550.026.07383516
GTACATG3134750.026.04878222
GAGTACA3136900.026.0319220
CAGTGGT3296400.025.8937424
GTATCAA3247550.025.750389
TGGTATC3294250.025.7207747
TACATGG3186100.025.63324423
AGAGTAC3205950.025.56463419
AGTACAT3185250.025.52849821
GGTATCA3307250.025.5274288
AACGCAG3277150.025.39472214
GCAGAGT3250250.025.30812617
Produced by FastQC (version 0.11.5)