FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_cDNA3P_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_cDNA3P_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81773557
Sequences flagged as poor quality0
Sequence length49
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8046310.9839745628284216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1541950.034.3289571
AGCAGTG1729650.030.5129282
TCAACGC1853500.027.68600712
ATCAACG1878400.027.49753611
CAACGCA1865750.027.47426213
GTGGTAT1919350.027.3391916
AGTGGTA1960200.026.8423715
TATCAAC1942050.026.65542210
CGCAGAG1947400.026.26099216
GAGTACA1944700.026.08006920
GTACATG1946400.026.02840822
CAGTGGT2031800.025.9721074
GTATCAA2004450.025.9544149
TGGTATC2034200.025.7770187
GGTATCA2031050.025.7577728
AGAGTAC1980300.025.67344919
TACATGG1975300.025.63087523
AGTACAT1977100.025.56113621
AACGCAG2021300.025.53143514
GCAGAGT2000750.025.46742217
Produced by FastQC (version 0.11.5)