FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_HTO3P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_HTO3P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26728563
Sequences flagged as poor quality0
Sequence length49
%GC16

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25648739.596000353629188No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24821559.286526178006651No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14838085.551394588627903No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13608625.091414753572797No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12335144.614965645553036No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11698844.376905709446483No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11300114.227728217188481No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11235214.203447076447769No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5902812.2084277407655626No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4801831.7965163334819008No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4105551.5360159840991077No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3926031.4688518795417471No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3409591.275635356827825No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2946941.102543372795612No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1743320.6522310982449748No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1412680.5285282265268058No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1393540.5213673477320873No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1368030.5118232506551138No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1224910.45827753628206647No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1224370.4580755052188927No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA882680.33023847933762845No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA869310.3252363398660826No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT847120.31693435969603007No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT809650.3029156487013537No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT789900.29552654963156827No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC721950.27010430751552184No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC719550.2692063916791935No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG699340.2616451920741119No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT691150.2585810542826414No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT678580.25387822008987165No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT636800.23824700190578896No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT622930.23305779663500803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG606120.2267686444647249No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG593580.22207703421990924No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT543940.20350514167185121No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG531030.1986751027356016No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG512310.19167135921224046No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC472330.17671357790540404No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC464820.17390384960089325No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC438880.16419887593657767No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT427300.15986643202629336No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT424200.15870662407103592No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC423470.1584335080041527No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC421730.15778251902281465No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG413430.15467722675551243No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG410730.15366707143964303No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT390260.14600859761895918No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC379890.14212885294282376No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG378830.14173227344844538No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT373480.13973066939663012No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT346340.12957673781415036No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG344150.12875738961350072No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG338360.1265911676583586No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA334540.12516198495220263No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT327740.12261789008260564No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT322810.12077342130214781No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT295180.1104361652364177No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA294950.11035011496876956No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT289050.10814273853779569No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG288480.10792948352666772No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG287020.1073832513929013No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA279220.10446502492483416No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG270600.10124001054602148No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG269930.1009893423750465No Hit
AGTAAGTTCAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA268880.10059650419665285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA255.0866234E-543.00697311
TCGCCCA1400.043.00697311
TCGCCAT351.7321145E-743.00697311
CTTCGAA351.7321145E-743.00697311
TGTCAGC650.043.00697311
TGTCACG503.6379788E-1143.00697311
TGTCAAG351.7321145E-743.00697311
CTATGAA6050.043.00697311
CCTTAGA302.960027E-643.00697311
TCGCATG1600.043.00697311
TCGCAGT1900.043.00697311
TCGCAGC1900.043.00697311
TCGCACC1000.043.00697311
TCGCAAC1000.043.00697311
CGTTGTA456.020855E-1043.00697311
TAGCGCT255.0866234E-543.00697311
TAGCGCG351.7321145E-743.00697311
CATTCTA850.043.00697311
TCCTAGA401.0184522E-843.00697311
CTTGGTA302.960027E-643.00697311
Produced by FastQC (version 0.11.5)