FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_GO3P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_GO3P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48698686
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGGAAAGGACGAAACACCGCGGGCCTCCAGAAAACATTGGTTTAA8126891.6688109408126535No Hit
CTTGTGGAAAGGACGAAACACCGATGATGCCAATGTCCTGGTGGTTTAA7675761.57617394440581No Hit
CTTGTGGAAAGGACGAAACACCGCAGCTGTAATCCAAGGAATGGTTTAA7630921.5669663037725494No Hit
CTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTTAACGGCGGTTTAA7620841.5648964327292114No Hit
CTTGTGGAAAGGACGAAACACCGCACCCTGCTGAGAAATGAAGGTTTAA7000551.437523386154608No Hit
CTTGTGGAAAGGACGAAACACCGACAGTTCAGAATTCATCTGGGTTTAA6913371.4196214657619306No Hit
CTTGTGGAAAGGACGAAACACCGTGTCCGAATCAAAACTTGTGGTTTAA6443411.3231178352532962No Hit
CTTGTGGAAAGGACGAAACACCGAGCTGAAGTCCTGTCATGTGGTTTAA6319011.2975729981708337No Hit
CTTGTGGAAAGGACGAAACACCGCCACATTTATAAGAGTTGCGGTTTAA6059391.2442614981439128No Hit
CTTGTGGAAAGGACGAAACACCGCGCTTCCGCGGCCCGTTCAAGTTTAA5761151.1830195993378547No Hit
CTTGTGGAAAGGACGAAACACCGTGTGAACTTCTCCTCTGTCAGTTTAA5757421.18225366491408No Hit
CTTGTGGAAAGGACGAAACACCGACGGTCAATGAGCACATACAGTTTAA5519811.1334617940204792No Hit
CTTGTGGAAAGGACGAAACACCGTCACAATGTACTTTATGTGGGTTTAA5471441.1235292878333514No Hit
CTTGTGGAAAGGACGAAACACCGGTTGCCGTAGAGCTTGCCCAGTTTAA5329041.0942882524592141No Hit
CTTGTGGAAAGGACGAAACACCGGACAGAGAGCAAGTTTTCTGGTTTAA5306671.0896946993600607No Hit
CTTGTGGAAAGGACGAAACACCGGAAGCAGCTGCGCTACACGGGTTTAA5276931.0835877584048161No Hit
CTTGTGGAAAGGACGAAACACCGGATTTTGCAGGTCCACTGCGGTTTAA5237631.075517725467993No Hit
CTTGTGGAAAGGACGAAACACCGAGAAGCCTCCCCCATACCTGGTTTAA5212401.0703368875291626No Hit
CTTGTGGAAAGGACGAAACACCGACCTAAACTGGATATCAGAAGTTTAA5188291.0653860352618139No Hit
CTTGTGGAAAGGACGAAACACCGTGAAGCCTGTGGATGTCGGGGTTTAA5185291.0647700022132014No Hit
CTTGTGGAAAGGACGAAACACCGCTCCCTGCCAGATATCGAGGGTTTAA4996961.0260975008648077No Hit
CTTGTGGAAAGGACGAAACACCGGCACTGCCTTGACAAATCAAGTTTAA4962261.0189720519358572No Hit
CTTGTGGAAAGGACGAAACACCGTTCCGGGCACGGCAGCGCCGGTTTAA4896431.0054542334058048No Hit
CTTGTGGAAAGGACGAAACACCGTGAGGGTATTGTCTCCTCCAGTTTAA4791520.9839115576958277No Hit
CTTGTGGAAAGGACGAAACACCGCAGTGAGCAACTGGTAATGGGTTTAA4669920.9589416847920702No Hit
CTTGTGGAAAGGACGAAACACCGCTTCAAGATGCCGACAAAAAGTTTAA4616650.9480029912922086No Hit
CTTGTGGAAAGGACGAAACACCGCAACCAGCCAGAAATTGCTGGTTTAA4584090.9413169792712682No Hit
CTTGTGGAAAGGACGAAACACCGAAGGCTTTCAGGTGGTAGGGGTTTAA4582060.940900130241707No Hit
CTTGTGGAAAGGACGAAACACCGACAGTACTGAGAATTCAGTGGTTTAA4518570.9278628174895726No Hit
CTTGTGGAAAGGACGAAACACCGGCTGGATTTTTTTCGGGTAGGTTTAA4447140.9131950706021102No Hit
CTTGTGGAAAGGACGAAACACCGAACCACGGCATTGAGAGGTGGTTTAA4444670.9126878700587527No Hit
CTTGTGGAAAGGACGAAACACCGCCATATCGGGGCGAGACATGGTTTAA4416230.9068478767579068No Hit
CTTGTGGAAAGGACGAAACACCGCCCTCCGGACGTCACCACGGGTTTAA4370770.8975129226279329No Hit
CTTGTGGAAAGGACGAAACACCGACGGAGGCTAAGCGTCGCAAGTTTAA4356840.8946524758388759No Hit
CTTGTGGAAAGGACGAAACACCGGGTGTACAGCCCCACTACGGGTTTAA4330010.8891430869407851No Hit
CTTGTGGAAAGGACGAAACACCGAGCTCTGCCGTCCTGCAAGGGTTTAA4314840.8860280131583017No Hit
CTTGTGGAAAGGACGAAACACCGAGGAGCCAGATCCCAAGACGGTTTAA4301060.8831983680216751No Hit
CTTGTGGAAAGGACGAAACACCGCCTTTGGTACAACATGCTGGGTTTAA4238270.8703047963142166No Hit
CTTGTGGAAAGGACGAAACACCGATGGCAGAGATCTGATGCAGGTTTAA4194330.8612819655955398No Hit
CTTGTGGAAAGGACGAAACACCGGCATCAATGAATCTAAAGTGGTTTAA4022050.8259052410572228No Hit
CTTGTGGAAAGGACGAAACACCGACACGTAATCCCACAGCAGGGTTTAA3955230.8121841316211285No Hit
CTTGTGGAAAGGACGAAACACCGAGATGTTTGGAGCTTTGGAGGTTTAA3876920.7961036156088482No Hit
CTTGTGGAAAGGACGAAACACCGGGACGTAGATGGAATAGACAGTTTAA3801550.7806268119842084No Hit
CTTGTGGAAAGGACGAAACACCGGACCTGTCACCACAACAAAGGTTTAA3785840.7774008522529746No Hit
CTTGTGGAAAGGACGAAACACCGTGGCCCTGAGCAATAGCACGGTTTAA3775130.7752016142694281No Hit
CTTGTGGAAAGGACGAAACACCGCTTTCTCCCGAGCGGCGCGGGTTTAA3737350.7674437047439021No Hit
CTTGTGGAAAGGACGAAACACCGTACTAACGCCGCTCCTACAGGTTTAA3566770.7324160655997988No Hit
CTTGTGGAAAGGACGAAACACCGAGGCTAAGTGTAGACACGTGGTTTAA3532040.725284456340362No Hit
CTTGTGGAAAGGACGAAACACCGTAAACACCTCAACGATGACGGTTTAA3503950.7195163335618542No Hit
CTTGTGGAAAGGACGAAACACCGGTATAAAATACTCACGCAGAGTTTAA3472960.7131527121696877No Hit
CTTGTGGAAAGGACGAAACACCGGAATAAAAAAAGCAAAGCCAGTTTAA3357750.6894949896594745No Hit
CTTGTGGAAAGGACGAAACACCGGGGTGCAGAGTCCGTGACAGGTTTAA3346760.6872382552580577No Hit
CTTGTGGAAAGGACGAAACACCGCAGGTGCCTTAGTGACCACGGTTTAA3344760.6868275665589827No Hit
CTTGTGGAAAGGACGAAACACCGTGTGCAACCGTACACGAAGGGTTTAA3316310.6809855198146414No Hit
CTTGTGGAAAGGACGAAACACCGGTAAGACCAAAGAAAAGGAGGTTTAA3314210.6805542966806127No Hit
CTTGTGGAAAGGACGAAACACCGGGGCAGAAGTTGCTGTCCTGGTTTAA3303920.6784413033238721No Hit
CTTGTGGAAAGGACGAAACACCGATAACAAAAAAAGGCCGTGGGTTTAA3239580.6652294478746306No Hit
CTTGTGGAAAGGACGAAACACCGCCGGCCGGAGACGCAAGAAGGTTTAA3198860.6568678259614644No Hit
CTTGTGGAAAGGACGAAACACCGGAATAGAGTTCAAGGACAAGGTTTAA3149990.6468326475995677No Hit
CTTGTGGAAAGGACGAAACACCGAGTGTACGACGCGCACACCAGTTTAA3129100.6425430041377297No Hit
CTTGTGGAAAGGACGAAACACCGGGCTTGAACGGATACTCCGGGTTTAA3108420.6382964829892946No Hit
CTTGTGGAAAGGACGAAACACCGCCCAGGTAGATGGTATAGCGGTTTAA3021260.6203986694836078No Hit
CTTGTGGAAAGGACGAAACACCGTCTACAGGACAACACTGGGGGTTTAA3014280.6189653659238362No Hit
CTTGTGGAAAGGACGAAACACCGAGCAGGATAGTCCTTCCGAGGTTTAA2999480.6159262695506815No Hit
CTTGTGGAAAGGACGAAACACCGGAAGCCTGCTGAGGGACTGGGTTTAA2992410.6144744849994516No Hit
CTTGTGGAAAGGACGAAACACCGTGCGGCGTTGCTTGAAGGTGGTTTAA2972730.610433308200554No Hit
CTTGTGGAAAGGACGAAACACCGTCAGGATAGAGATAGACCAGGTTTAA2954040.6065954223076984No Hit
CTTGTGGAAAGGACGAAACACCGACGCTCCTCTTTCTCCCGAGGTTTAA2878010.5909830914123637No Hit
CTTGTGGAAAGGACGAAACACCGGCTAAAGTCTCCATAGACAGGTTTAA2841130.5834099918014215No Hit
CTTGTGGAAAGGACGAAACACCGACCTGAAACCATTCACCACGGTTTAA2793520.5736335473199421No Hit
CTTGTGGAAAGGACGAAACACCGACAGCTGAAACAGCTTCTTCGTTTAA2782690.5714096680144511No Hit
CTTGTGGAAAGGACGAAACACCGGACCTAAAATCATTAACATCGTTTAA2560720.5258293827476166No Hit
CTTGTGGAAAGGACGAAACACCGCTTCGGGGAGACAACGACGGGTTTAA2537540.5210695007253379No Hit
CTTGTGGAAAGGACGAAACACCGGGATACGTGGACCCTTCTGGGTTTAA2524510.5183938638508645No Hit
CTTGTGGAAAGGACGAAACACCGTAAACTGGATATCAGAAAGGGTTTAA2508660.5151391559106955No Hit
CTTGTGGAAAGGACGAAACACCGTGGGGGCAAATACGTAGACTGTTTAA2312140.4747848843395898No Hit
CTTGTGGAAAGGACGAAACACCGTACATGGTATAGTGTTTATTGTTTAA2278200.4678154971162877No Hit
CTTGTGGAAAGGACGAAACACCGGACTCCGGGAAAGATGGTCCGTTTAA2216170.45507798711447783No Hit
CTTGTGGAAAGGACGAAACACCGGATAGAAGTGCGAGAGAAGGGTTTAA2212900.4544065110914902No Hit
CTTGTGGAAAGGACGAAACACCGTAAGACCCAGATCCAGTCCGGTTTAA2210980.45401224994037825No Hit
CTTGTGGAAAGGACGAAACACCGCCAGGCTCAACATTAGCAGGGTTTAA2113360.43396653453852946No Hit
CTTGTGGAAAGGACGAAACACCGCAAGTGCAAGGTGTACTGGGGTTTAA2035390.41795583560509214No Hit
CTTGTGGAAAGGACGAAACACCGGGCACGAGACATCTTCTCGTGTTTAA2000560.4108036919107017No Hit
CTTGTGGAAAGGACGAAACACCGCAGTTGGAATGTAAAGGTGAGTTTAA1928960.39610103648381806No Hit
CTTGTGGAAAGGACGAAACACCGTGTCCTGTAGAGGGTCATACGTTTAA1895760.38928360407917373No Hit
CTTGTGGAAAGGACGAAACACCGGCGCAACAAGAAGGTCCCGTGTTTAA1836610.3771374858040318No Hit
CTTGTGGAAAGGACGAAACACCGTGTTCTGGAATACTCACGTGGTTTAA1795950.3687881845518378No Hit
CTTGTGGAAAGGACGAAACACCGCAGCTGAAGTTTCTGCCAGGGTTTAA1743400.3579973389836432No Hit
CTTGTGGAAAGGACGAAACACCGCATGTTGTACCAAAGGATGGGTTTAA1730230.35529295390023463No Hit
CTTGTGGAAAGGACGAAACACCGGCTGTGGTCATCAGGCAGAGGTTTAA1640820.3369331156080885No Hit
CTTGTGGAAAGGACGAAACACCGCTTACCTGGATATAGTCAACGTTTAA1621850.33303773329736247No Hit
CTTGTGGAAAGGACGAAACACCGGTAGGCGCGCCGCTCTCTACGTTTAA1588630.3262161940057274No Hit
CTTGTGGAAAGGACGAAACACCGGCCGACAAAAAAGGAAAGTCGTTTAA1584490.3253660683986422No Hit
CTTGTGGAAAGGACGAAACACCGCCATCATAGCCCTTAAATCCGTTTAA1464800.30078840320250116No Hit
CTTGTGGAAAGGACGAAACACCGAGACCTGGTGCCCTATGACAGTTTAA1370120.28134640018829254No Hit
CTTGTGGAAAGGACGAAACACCGGCCGTATTTCTACTGCGACGGTTTAA1232450.2530766435874676No Hit
CTTGTGGAAAGGACGAAACACCGAAAGTATTACTCCAGGCCAAGTTTAA1220310.2505837631840826No Hit
CTTGTGGAAAGGACGAAACACCGTCAATGAATCTAAAGTGCGGGTTTAA1207370.24792660730106764No Hit
CTTGTGGAAAGGACGAAACACCGCGAAGCGGGAAATGGTCGTCGTTTAA1181420.24259792143057005No Hit
CTTGTGGAAAGGACGAAACACCGAGCAACATGAGATTGAATCCGTTTAA1078200.2214022776713113No Hit
CTTGTGGAAAGGACGAAACACCGGTAGGGAGAATAGCCCTGGTGTTTAA1006700.20672015667938143No Hit
CTTGTGGAAAGGACGAAACACCGTGGGTACTCGTCCTCCTTGCGTTTAA982420.2017343958726114No Hit
CTTGTGGAAAGGACGAAACACCGTCGCCGTCAGACACCTCCAGGTTTAA814370.16722627793283787No Hit
CTTGTGGAAAGGACGAAACACCGTGTTACTGTTGATGGATACGGTTTAA685670.14079846014736414No Hit
CTTGTGGAAAGGACGAAACACCGTCTCGAACTCGCTGTGCACGGTTTAA665710.13669978693059603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648530.13317197100554212No Hit
CTTGTGGAAAGGACGAAACACCGGCACGTCCTCGATACCCCCAGTTTAA533070.10946291240794463No Hit
CTTGTGGAAAGGACGAAACACCGGCTAAAGTCTCCATAGCAAGGGTTTA507310.10417324196385916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC650.043.00522220
ATCGCTC255.0879076E-543.00522220
CGGATCT208.7965076E-443.00509322
CTATCGT1100.043.0050922
CTATTAT351.7327329E-743.0050922
CGATCTT2550.043.0050922
GATCGCT208.797308E-443.00429523
GGGCTCT351.7329876E-743.00429523
GGACTCT302.9613439E-643.00429523
GATCTAT302.9613439E-643.00429523
AAGCTCT456.020855E-1043.00429523
CTTCCCT1400.043.0033724
ATTACCT650.043.0033724
ATCGTTC208.798242E-443.0033724
GTTACCT208.798242E-443.0033724
TGTCTGA503.6379788E-1143.00336524
CTAGTAG503.6379788E-1143.002571
TCGTTAC1350.043.00217425
ACTAGCG302.962348E-643.00217425
TGATTAC208.7994436E-443.00217425
Produced by FastQC (version 0.11.5)