FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_ADT3P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_ADT3P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46051982
Sequences flagged as poor quality0
Sequence length49
%GC18

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495035910.749502594698313No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493381010.713567116394685No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27526345.977232424002945No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12786902.776623164666398No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12587392.7333003821637907No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9496762.0621826873814033No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9483512.059305503941177No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8035991.7449824417980535No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7109471.5437924039838287No Hit
ACTCACTGGAGTCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6333671.3753305992345781No Hit
ACTCACTGGAGTCTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5430271.179160975091148No Hit
ACGAATCAATCTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4909101.0659910359558467No Hit
ACTCACTGGAGTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4712181.023230661386083No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4112610.8930364821214426No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3951700.8580955321314943No Hit
TGATAGAAACAGACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3603750.7825396092615514No Hit
GATGAGAACAGGTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3547100.7702382928925838No Hit
TGATAGAAACAGACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3292520.7149572845746357No Hit
GATGAGAACAGGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3114630.6763291968627974No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3094780.6720188503504583No Hit
GACTGGGAGGGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020780.6559500522691943No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3003250.652143484291295No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2932620.6368064679604887No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2931550.6365741218260704No Hit
TGATAGAAACAGACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2719630.5905565584560508No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2661230.5778752367270533No Hit
ACGAATCAATCTGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2582810.560846653679314No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2578500.5599107547640403No Hit
GTGCATTCAACAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360090.5124839143731099No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2218480.48173388063949124No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2203670.47851794956403837No Hit
ACGAATCAATCTGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2068850.4492423366273356No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2039470.4428625894972338No Hit
GATGAGAACAGGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1962690.4261901257583224No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1950500.42354311699331415No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1903400.41331554416050975No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1852050.40216510116763265No Hit
GTGCATTCAACAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1526100.3313863885380655No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1332230.28928830902435426No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1269360.2756363450328805No Hit
GTGCATTCAACAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1259640.27352568669031446No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1156660.2511639998469556No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1124330.24414367225280334No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1029610.22357561070878557No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA982400.2133241518247792No Hit
GTCTTTGTCAGTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA969770.2105815988549635No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG962480.208998605098039No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC952300.20678805963226513No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA951980.20671857293785967No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA939760.20406504979525092No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT931310.20223016677110664No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT912900.19823251038359216No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT773410.16794282600040972No Hit
GTCTTTGTCAGTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA747260.16226446019196308No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT714370.1551225308826013No Hit
GTCTTTGTCAGTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA713390.1549097278809846No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA703520.152766497650416No Hit
TCAACGCTTGGCTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA678420.1473161350579873No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA669180.14530970675702948No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG666990.1448341571921921No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG658660.1430253316784498No Hit
TCAACGCTTGGCTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA648920.14091033041748344No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC634720.1378268583532409No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG633510.13756411179002023No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT613100.13313216356247165No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT611780.1328455309480491No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT597770.1298033166086098No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC574730.12480027461141628No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA573550.12454404242579614No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA572780.12437684006738298No Hit
CTCTATTCAGACCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG534500.11606449424912918No Hit
TCAACGCTTGGCTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA531570.11542825670347913No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG515880.11202123721841115No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT513350.11147185804076794No Hit
CTCTATTCAGACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT495600.10761751796046477No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG490740.10656218878918175No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA489270.10624298428675664No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT461120.10013032663827585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAT351.7320599E-743.0072414
CTCGATA850.043.007113
CTCGACA255.086592E-543.007113
TCGCTAA750.043.0069612
TCTCGAG600.043.0069612
TCTTCTA255.0866904E-543.00695812
TCTTCGA456.020855E-1043.00695812
TCTTCCA255.0866904E-543.00695812
CCTACTA800.043.00695812
TCTCGAT850.043.00695812
TCTCGAC255.0866904E-543.00695812
TCTCCTC255.0866904E-543.00695812
TCTCCAT1050.043.00695812
TCTCCAG800.043.00695812
TCTCCAC401.0184522E-843.00695812
GTCTCTT1700.043.00672511
ACCTACT850.043.00672511
GTGTCCA255.0868533E-543.00672511
CTGTTGT208.7948574E-443.00672511
TACCTGA4350.043.00672511
Produced by FastQC (version 0.11.5)