FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26894864
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG4680811.7404103623650968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCC59000.017.5748148
TGCGGGT35900.017.2280461
ATGCGAT43200.016.9577941
GGGACCT26400.016.907251
ACGAGGA36150.016.832061
TGTCGAG54150.016.804376
CCGGGAT31650.016.6639861
GGCTCGA39200.016.5181061
ATGGGAG41700.016.48781
GCGCGAT26900.016.4813751
GACTAAC42100.016.402461
GGGAATG31200.016.3269561
CGGACGT29550.016.3241861
GCTGCAG34800.016.2771821
CTCGGGA34400.016.2628041
TGGCCAG40900.016.0517561
GTGTCGA56650.015.93934255
CCGGTAG37400.015.9216241
CTCGAAA37800.015.9119981
ACGAGCC29750.015.9116861
Produced by FastQC (version 0.11.5)