FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_cDNA3P_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_cDNA3P_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86112633
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA272450.017.68348
TGACGGC210350.017.5362321
ATGCGAT267950.017.4881441
GGCGACT218650.017.3097021
ACTGAAC252550.017.2872071
TTGAACG253400.017.158171
GGCTCGA252650.017.0705451
TGAGCCG174050.017.0157971
GACTAAC231750.016.9095731
GGAAAGC205900.016.904831
CCGGGAT184700.016.8956781
CCGGTAG199950.016.8376161
CGGACTG170650.016.7335381
GGGAATG183750.016.7108671
GGGACCT156450.016.679631
CCGTGGA198150.016.6574211
CGCTGGA187400.016.6469141
ACGAGGA170000.016.6445451
GCCGAAG133950.016.6331843
GAATGAA226800.016.6127091
Produced by FastQC (version 0.11.5)