FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_cDNA3P_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_cDNA3P_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81872878
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA260100.017.7232828
TGACGGC192550.017.6614071
ACTGAAC238600.017.4931431
ATGCGAT251150.017.3796671
TTGAACG243850.017.3544241
GGCGACT206300.017.1969681
CCGGGAT184850.016.979441
GGCTCGA240550.016.9646431
GACTAAC217500.016.9322411
CCGTGGA185800.016.8118761
CGGACTG163400.016.7966231
GGATTAC239550.016.7390121
GGAAAGC200900.016.738161
TGAGCCG164850.016.7277581
ACGAGGA159550.016.7254941
GCCGAAG122700.016.6829153
CCGGTAG188850.016.6621741
CGCTGGA188250.016.6515221
GATGCTA219550.016.5782451
AGGCGAA101300.016.57403610
Produced by FastQC (version 0.11.5)