FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_cDNA3P_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_cDNA3P_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132843414
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA419950.017.6776568
ATGCGAT403150.017.5948561
TGACGGC310600.017.5500641
ACTGAAC398000.017.4330081
GGCGACT326450.017.2647761
TTGAACG391000.017.2462921
GACTAAC357400.017.1773451
GGCTCGA391250.016.9642931
CCGGGAT290200.016.9122871
TGAGCCG271100.016.7461261
CCGGTAG313500.016.7304971
GATGCTA358050.016.707591
GGGACCT240800.016.6435741
CGGACTG268400.016.6090151
CCGTGGA301100.016.5956921
GGAAAGC316900.016.5951821
GGATTAC396850.016.5736871
GAATGAA353450.016.5713081
ACGAGGA258950.016.5700991
GGGAATG283100.016.548581
Produced by FastQC (version 0.11.5)