FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_cDNA3P_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_cDNA3P_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81773557
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC190550.017.6951221
ATGCGAT252900.017.6198671
CAATGGA253900.017.4865658
ACTGAAC239250.017.4294511
TTGAACG240350.017.270611
GGCGACT206750.017.1987881
GACTAAC219950.017.0579511
GGCTCGA240500.016.9520741
TGAGCCG169600.016.9439051
CCGTGGA183650.016.785931
CCGGGAT171600.016.763911
GGGACCT152650.016.7154241
GGGAATG179100.016.6818161
GGAAAGC197600.016.6487121
ACGAGGA168650.016.6360511
CCGGTAG190750.016.6225811
GAATGAA214850.016.6109031
CGGACTG166350.016.5654221
GATGCTA212650.016.5334591
CTGCGGA141900.016.529671
Produced by FastQC (version 0.11.5)