FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_HTO3P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_HTO3P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26728563
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGC1500450.019.8631327
TCGAGCC1512450.019.795498
TGTCGAG1508400.019.7678036
TGTGTCG1520600.019.6808854
GTGTCGA1515000.019.6764075
GTGTGTC1532300.019.6345583
CGAGCCC1522100.019.5899129
CTCAATA1434800.019.5516936
TGTGTGT1540000.019.551552
ACTCAAT1437050.019.5336075
ATGTGTG1577600.019.5236451
CAATAAG1448700.019.5173348
AATAAGC1448350.019.498659
TCAATAA1444150.019.4977767
CTTACTC1446500.019.4429952
ATAAGCA1454800.019.39505210
CAGAATC1071800.019.3690361
GAGCCCA1539000.019.36638310
TACTCAA1447500.019.3594264
TTACTCA1455700.019.3405463
Produced by FastQC (version 0.11.5)