FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n01_GO3P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n01_GO3P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48698686
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACGA23450.019.7867266
AAACAAA46750.019.7646715
AGGCCGA46800.019.6146288
GCGGGCG11800.019.5764618
GCGGGCA19550.019.5394515
GGGAAAC15950.019.497689
AATAGGA17500.019.428545
GTGATGT74750.019.4248
GCAGACC20750.019.4216356
TCAGGAG31900.019.4045773
AAGGCGA17700.019.3777837
CGGACGT101050.019.3109111
CGCACGA22950.019.302786
GACGGGA12300.019.26810315
CGACTGG6250.019.19981215
AACGTAA67750.019.1881625
TGCGGGT129600.019.1857131
TATGGCG9250.019.13537820
GAGCGGG8000.019.12475214
TGCGACG37800.019.07339510