FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37209808
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589070.15831041106151367No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT423750.11388126485361065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA85600.024.5418978
ACTACCC92800.023.4176436
CTAGTTG125250.023.0797398
ACTCTAG150800.020.5631245
TACTCTA146300.020.3931564
TCTAGTT158300.019.6992537
CTAATGC143150.018.63020121
ACCTCTA146450.018.56260117
CTCTAGT167000.018.2387396
GGACACC131900.018.04947929
CCTACAC122550.017.70641111
CTACCCT120650.017.6408737
TACTACC121150.017.538325
GACACCT139050.017.24557530
GTTACCT161550.016.95541814
GCTGGAC150900.016.59965326
TTGTACT176400.016.3856371
ATGCTGG160800.016.32814224
TACCTCT173100.016.00389716
TAGTTGT191050.015.9436239
Produced by FastQC (version 0.11.2)