FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10036922
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC248070.2471574452805352No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT218250.21744714166354986No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA208080.20731455320665038No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT185840.18515636566668547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166210.1655985769342434No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA162330.16173284997133583No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACGT138830.13831929748980812No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT122870.12241800823001314No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT116210.11578250782460998No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT105230.10484289904813449No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCG103740.1033583801886674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT49450.039.9664271
ACTCTAG78250.033.7658275
GACTCTA66750.031.313114
CTAGCAG65900.030.2554448
CTGACTC66700.029.8880922
TGACTCT82400.024.905463
CTCTAGC82200.024.8846196
TAGCAGA84500.023.8819039
CTAGTTG28650.023.2377348
TACCCTA18200.022.3651458
CTATCGA89300.022.25310317
TCTATCG89900.021.9510816
ACTACCC20200.020.3209366
ATCTATC100700.020.04242515
GATCTAT102300.019.52573414
GCAGATC101550.019.4657211
TACTCTA34650.019.3109364
GGACACC26450.019.3056229
ACCTCTA33050.019.20728117
CTAATGC33300.019.16754721
Produced by FastQC (version 0.11.2)