FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30258128
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG561770.18565920535467362No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA390330.1290000491768691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA58700.022.5072788
ACTACCC66300.020.186376
CTAGTTG84200.018.9684228
ACTCTAG106600.016.4050065
TACTCTA100250.016.3077134
CCTACAC87700.015.61669411
TCTAGTT115400.015.4541667
ACCTCTA97950.015.28596317
GCCGGGT33350.015.0994251
TACTACC88900.015.0929285
GCGGGCT21550.014.88466551
GGACACC90350.014.81718429
CTCTAGT119000.014.3192426
CGGGGAT269000.014.1296941
CTAATGC104700.014.00466621
CGGGCAT22500.013.9496171
CCGGGGT23700.013.8254311
CTACACT98600.013.68042112
GTACTCT121000.013.5963643
TAGCCTA77250.013.488090542
Produced by FastQC (version 0.11.2)