FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685097
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281410.20563244820259585No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT147860.10804453925317445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA37750.028.966738
ACTACCC39600.028.1361396
CTAGTTG48200.024.117938
ACTCTAG56200.023.0778855
TACTCTA54700.022.8270534
CCTACAC50600.021.543811
CTACCCT51950.021.2482517
ACCTCTA54150.021.2159917
TACTACC53200.020.8131545
TCTAGTT62000.020.6971247
GGACACC49800.020.36933129
CTCTAGT61550.020.3439736
CTAATGC56700.019.5925221
CTGTTAC58650.019.2314971
GTTACCT59650.019.02787414
GACACCT55150.018.26828230
TACCTCT65000.017.83397716
CTACACT60950.017.82901612
TTGTACT66650.017.6476781
GCTGGAC59550.017.61305626
Produced by FastQC (version 0.11.2)