FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10181107
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC274660.2697742003890147No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT226950.2229128914959837No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA215320.21148977218292667No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204810.20116672970827237No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT190050.18666928851646486No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA163850.16093534818954364No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACGT151670.14897201257191384No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT129560.12725531712808835No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT121500.11933869273744004No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT109360.10741464557832464No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCG102340.10051952110905034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT47950.039.8477021
ACTCTAG79750.033.8244485
CTAGCAG65050.029.9628818
GACTCTA68600.029.5138594
CTGACTC66150.029.5109832
CTCTAGC78750.025.1877336
TGACTCT83350.023.9181653
TAGCAGA87150.022.4836929
CTAGTTG33400.021.6897878
TACTCTA34350.021.4881524
CTATCGA90500.021.46188517
TACCCTA23450.021.3308228
ACTACCC23700.021.1052936
TCTATCG91300.021.0846916
ACCTCTA35800.019.3696217
TCTAGTT41100.019.0531377
GGACACC33600.018.68959629
GTTACCT37300.018.68289614
GATCTAT104000.018.6093514
ATCTATC104750.018.47628815
Produced by FastQC (version 0.11.2)