FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21140229
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339030.1603719619120493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA76050.027.4415938
ACTACCC82950.025.7820346
CTAGTTG87800.024.4370638
TACTCTA94450.023.62674
ACTCTAG100400.022.6401965
TCTAGTT104550.021.9076187
CTACCCT96250.021.8615117
CCTACAC97750.021.63374911
TACTACC98350.021.6040335
CTCTAGT106350.020.9201156
GTACTCT110250.020.3966313
ACCTCTA103000.019.82799117
TGTACTC112750.019.424312
CTGTTAC111200.019.415691
GGACACC95300.019.29784229
CTAATGC105500.019.098321
CTACACT112150.018.61012612
GTTACCT110850.018.57940914
GACACCT100900.018.4663130
TTGTACT114800.018.4463211
Produced by FastQC (version 0.11.2)