FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20424684
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT506790.24812623784044838No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA339060.16600501628323847No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT248380.12160775657532817No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG223970.10965653128342157No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACATGAACTGAGGGGACAGGATGTCCCAGGCGA204980.10035895781790308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA152400.035.713644
ACTCTAG162950.033.5724645
TTGTACT164250.033.0955241
CTCTAGT166300.032.95926
GTACTCT170600.032.0855833
TCTAGTT173100.031.7453987
TGTACTC174300.031.2853642
CTAGTTG172900.031.104018
ACTACCC144200.028.8009136
GTTACCT185650.028.3754214
TAGTTGT192400.028.1674169
ACCTCTA191350.027.6207817
TACCCTA148850.027.5780378
CTACCCT151250.027.4595327
CTAATGC191750.027.09657721
TACCTCT203500.026.141416
CCTACAC156500.026.09932111
TTACCTC203250.026.08881215
CTGTTAC163000.025.773781
CCTCTAA204350.025.71214918
Produced by FastQC (version 0.11.2)