FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8561382
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT202940.23704116928785565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158830.18551911361974036No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA131600.15371350092777078No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG91700.10710887564647857No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACATGAACTGAGGGGACAGGATGTCCCAGGCGA86300.10080148275126609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA60800.032.334324
ACTCTAG63250.030.866075
CTCTAGT66500.029.4111656
GTACTCT67950.028.9307773
TCTAGTT68150.028.700097
CTAGTTG67800.028.441558
ACTACCC59300.028.3285986
TGTACTC70550.027.4731772
TACCCTA62050.026.7963968
TTGTACT72000.026.6326541
CTACCCT63150.026.3293277
CTGTTAC65100.025.9059811
CCTACAC63550.025.67672211
TAGTTGT77250.025.2308149
GTTACCT74700.025.16863814
ACCTCTA74800.025.13631217
TACTACC68750.024.583795
CTAATGC75400.024.5716921
GTTACTA69700.024.0479583
TTACCTC81350.023.36619615
Produced by FastQC (version 0.11.2)