FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12595966
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182330.14475269304474148No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT152460.12103875161301643No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA129370.10270748587285802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG93450.029.5648235
TACTCTA70600.028.1395664
CTAGTTG74050.026.3192238
TTGTACT79300.024.6182211
GTACTCT80950.024.5412463
CTCTAGT82500.024.5008586
TCTAGTT82400.024.4056857
ACCTCTA80200.023.8308917
TGTACTC82850.023.5616842
GTTACCT85850.022.46317914
GTGCGCA51100.022.21274868
CTAATGC85900.022.17064921
ACTACCC57300.021.9121196
GGACACC75550.021.87600729
TACCCTA56650.021.7988118
CTACCCT59400.021.1380357
GGTGCGC55300.020.900267
TAGTTGT96550.020.8292949
CCTCTAA92800.020.70708718
TGCGCAT54800.020.65002469
Produced by FastQC (version 0.11.2)