FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13268850
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238380.17965385093659209No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT154850.11670189956175553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA64000.024.8984784
CTAGTTG63850.024.3652388
TACCCTA49000.023.5128578
ACTCTAG70000.023.3567495
TCTAGTT71700.022.9964317
ACTACCC52650.022.3410116
GTACTCT73900.021.9356823
CTCTAGT74500.021.7151786
ACCTCTA71150.021.48050317
CTACCCT54200.021.3841367
TTGTACT76100.020.6679571
TGTACTC77350.020.600732
GTTACCT74650.020.4264714
CTAATGC74850.020.23438521
GGACACC67100.019.4884929
GCTGGAC74100.018.67115826
CCTACAC62300.018.60525911
ATGCTGG78900.018.54012524
TACTACC64100.018.5111125
TACCTCT82850.018.44690516
Produced by FastQC (version 0.11.2)