FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11648389
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160860.13809634963255435No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT130080.11167209474202827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG65850.030.2256228
TACTCTA67800.029.7075964
ACTCTAG71300.029.0263675
TCTAGTT73050.028.4742537
CTCTAGT76150.026.8162276
TGTACTC76900.026.2807032
GTACTCT77450.026.2284493
ACCTCTA73750.026.19557217
GGACACC69400.025.50586129
TTGTACT78900.025.0473611
GTTACCT78450.024.7134914
GACACCT71550.024.49831230
CTAATGC79000.024.36906421
GCTGGAC75700.023.88313726
TACCTCT82700.023.31920616
TAGTTGT89100.023.1523729
ATGCTGG80750.022.94439124
TTACCTC85250.022.90495915
CCTCTAA85300.022.73043818
ACTACCC54550.022.7010336
Produced by FastQC (version 0.11.2)