FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363455
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA164380.22323759702476623No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141470.19212448504132912No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT133960.18192546841122814No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC115930.15743968015014692No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA114930.1560816220103199No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT106900.1451764151475089No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA101880.1383589632855772No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT100290.13619965084325225No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT76570.10398651176655523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG74550.037.8014075
TACTCTA52750.037.6625754
CTAGTTG55850.035.2048848
TTGTACT56450.034.9512441
CTCTAGT57100.034.735556
TCTAGTT58150.034.2274367
TGTACTC58200.034.076492
GTACTCT58750.033.9918443
GTTACCT59350.032.4936414
ACCTCTA60350.031.72721117
TAGTTGT62850.031.4498319
ACTACCC44750.030.3700566
TACCTCT64600.030.01332716
TTACCTC64900.029.8749915
CTAATGC63950.029.34852821
CCTCTAA65650.029.06014318
TACCCTA46950.029.0210258
TGTTACC67550.028.95874613
CCTACAC46200.028.89856711
CTACCCT47600.028.769477
Produced by FastQC (version 0.11.2)