FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5568672
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT119760.21506025134897513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87260.1566980421903104No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA75200.13504117319174122No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACATGAACTGAGGGGACAGGATGTCCCAGGCGA61700.11079840938737279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA28750.029.514344
CTCTAGT31550.026.896456
ACTCTAG32350.026.763755
ACTACCC22600.026.4056516
GTACTCT32200.026.2444953
CTAGTTG32100.026.0062278
TCTAGTT33700.025.2835467
TACCCTA23300.024.7245038
CTACCCT24500.023.936127
TGTACTC36450.023.5640162
GTTACCT34600.023.53002714
TTGTACT37200.022.8126011
ACCTCTA36250.022.46021517
TAGTTGT38450.022.160299
CCTCTAA37350.021.52182818
CCTACAC25200.021.49262811
CTAATGC37350.021.24548721
TTACCTC38350.021.2297515
TACCTCT39400.020.8396616
GGACACC34500.020.60197329
Produced by FastQC (version 0.11.2)