FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9555312
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT191830.2007574425617918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185850.19449914351305325No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA131030.13712791377194172No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT108250.11328777124179723No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG96610.10110606540110884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA77700.031.5178814
ACTCTAG79950.030.6754725
TTGTACT79150.030.5936221
CTCTAGT81400.030.1299926
TCTAGTT84150.029.2687877
CTAGTTG82800.028.9129228
GTACTCT86300.028.3770483
TGTACTC87650.028.0587732
GTTACCT87700.026.3944814
TAGTTGT93650.025.821689
ACTACCC62250.025.600976
ACCTCTA91700.025.46979117
CTAATGC92200.024.92125121
TACCCTA63150.024.799648
CCTCTAA94200.024.68423318
GACACCT87950.024.4018130
TTACCTC97200.024.38365615
CTACCCT65150.024.1970467
GGACACC89750.023.98942829
TACCTCT99550.023.77351816
Produced by FastQC (version 0.11.2)