FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12536273
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160130.12773333828961767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128350.10238290120197606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG74450.026.176925
CTAGTTG53900.024.575458
TACTCTA55200.023.368884
TACCCTA44650.022.0182328
TCTAGTT63350.021.8350947
ACTACCC47150.021.2894846
GTACTCT65600.020.715613
CTCTAGT65800.020.7073766
CTACCCT49100.020.163127
ACCTCTA63650.019.7292517
TGTACTC69200.019.6376062
TTGTACT69350.019.1987931
GGACACC57850.019.14503129
CTAATGC65050.019.0397721
GTGCGCA45200.018.47244568
GTTACCT67000.018.38227714
GGTGCGC46500.018.03035467
CCTACAC55200.017.99837511
GACACCT62400.017.91506230
TGCGCAT48600.017.53488569
Produced by FastQC (version 0.11.2)