FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11083199
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150920.13617007147485125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA45100.026.7701248
ACTACCC46100.026.1136596
CTAGTTG50350.025.3490168
CTACCCT48300.024.9250057
TACTCTA54450.024.1342094
CCTACAC52950.022.8034311
ACTCTAG57850.022.7767315
TCTAGTT59550.022.3015947
TACTACC56700.021.47455
TTGTACT61100.020.8872931
CTCTAGT63150.020.811096
TGTACTC64400.020.0835082
ACCTCTA60650.020.07899517
GTACTCT66350.019.9615523
TACACTC60200.019.94300713
CTACACT60400.019.64814412
CTGTTAC63050.019.4754071
CTAATGC63250.018.9824421
GTTACCT65400.018.88423714
CACTCAT64100.018.5140115
Produced by FastQC (version 0.11.2)