FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33342407
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG649710.19485995717105847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA126800.024.5114178
CTAGTTG170750.023.7983848
TACTCTA175150.023.6311284
ACTACCC136200.023.3002836
ACTCTAG185950.022.9646225
TCTAGTT197950.021.8003127
CTACCCT144400.021.6671097
ACCTCTA187150.021.2732417
CTCTAGT199850.021.2645916
GTACTCT200300.020.9045733
TTGTACT197050.020.5849931
CCTACAC154650.020.29945211
TGTACTC204300.020.1913972
GGACACC176000.020.09422129
TACTACC157950.019.8945275
CTAATGC198250.019.7000421
GTTACCT205350.019.30305314
GCTGGAC197850.018.76410126
GACACCT189400.018.70919230
ATGCTGG205450.018.67393324
Produced by FastQC (version 0.11.2)