FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12211086
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC195500.16010042022470403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180420.14775098627591354No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT180210.14757901139996885No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA172380.1411668053111738No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG145720.11933418534600446No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT137360.11248794742744421No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT132710.10867993231724025No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA124640.10207118351307984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG108950.036.1558075
TACTCTA76250.032.6591224
ACTACCC73200.032.139116
CTCTAGT79150.031.5535496
TACCCTA75300.030.6480168
CTACCCT76900.030.189787
CTAGTTG81850.029.8812548
GTACTCT85000.029.0121573
TCTAGTT87100.028.9514587
CTGTTAC84600.028.0513461
TGTACTC88250.028.0225772
GCTGACT56250.027.8399831
TTGTACT88050.027.6965521
GTTACTA86900.027.1474653
TACTACC86900.027.0312775
CCTACAC85700.026.64853311
TAGTTGT93500.026.4536679
GACTCTA61750.025.6379264
ACCTCTA92600.025.59484917
GTTACCT91400.025.5911814
Produced by FastQC (version 0.11.2)