FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7953830
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT137430.17278468360525684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129890.16330497383021764No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA86700.10900408985356741No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT83640.10515688668226503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA54300.035.2537084
CTCTAGT57050.033.4983256
ACTCTAG59400.032.8687065
GTACTCT59900.032.0144423
TTGTACT59450.031.7368261
TCTAGTT61450.031.6057767
CTAGTTG61600.030.8567968
TGTACTC65000.029.7684142
GTTACCT65150.028.22356214
ACCTCTA65000.028.02402717
TAGTTGT71350.027.0271639
CTAATGC67850.026.08518421
CCTCTAA70050.025.95514118
TGTTACC72150.025.9159713
TACCTCT71600.025.4891516
TTACCTC72150.025.48611315
ACTACCC48200.025.406826
TACCCTA49950.024.2410568
GGACACC65900.024.2407829
GCTGGAC68700.024.10581626
Produced by FastQC (version 0.11.2)