FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25444364
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG521340.20489409756911195No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT308280.12115846165382638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC116500.030.468016
TACCCTA115500.030.2548398
CTACCCT129300.027.2657347
TACTCTA130900.027.0082284
CTCTAGT135900.026.1694016
ACTCTAG138500.026.0008415
CCTACAC138800.025.2771411
CTGTTAC142400.025.1906071
CTAGTTG139450.025.0092128
TCTAGTT151750.023.9594447
TACTACC152300.023.46375
GTACTCT155200.022.9790553
GTTACTA158250.022.5361753
CTACACT156250.022.299912
TTGTACT157100.022.0650631
TGTACTC160300.021.9465222
GTTACCT153700.021.83973514
ACCTCTA155700.021.5817817
TACACTC161750.021.43579313
TTACTAC169350.020.9779934
Produced by FastQC (version 0.11.2)