FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5533742
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151540.2738472447757774No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG68400.12360532890763609No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT56750.10255266689339691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA39950.033.15384
ACTCTAG47800.032.5458075
TTGTACT40850.031.3259811
TACCCTA35350.031.2270168
ACTACCC35850.031.0796016
CTAGTTG40850.030.907158
CTCTAGT43300.030.4324936
GTACTCT44900.029.2688313
CTGTTAC38300.029.0888521
TCTAGTT44900.028.9644267
CTACCCT38250.028.8594767
TGTACTC45700.028.3018172
TACTACC40050.028.0776965
GTTACCT46050.026.96982614
ACCTCTA46000.026.77390517
CCTACAC41450.026.29930511
CTAATGC47950.025.47154221
GTTACTA43250.025.3610823
TTACTAC43700.025.3362584
TAGTTGT52550.024.4851139
Produced by FastQC (version 0.11.2)