FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10167624
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240440.23647609313641021No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG232830.22899155200861088No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT218590.21498631341992977No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC135070.13284322866384515No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT133550.13134828746617697No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC121340.1193395821875396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT122850.039.813381
TACTCTA133200.037.002624
ACTCTAG136450.036.2248885
TGTACTC137650.035.6311652
GTACTCT141000.034.931723
TCTAGTT143700.034.6149947
CTCTAGT143700.034.3746076
CTGTTAC111250.034.3532451
CTAGTTG144550.033.7671288
ACTACCC113450.033.5672766
CTACCCT119400.031.6637277
TACTACC121350.031.4944745
TACCCTA120450.031.273158
GTTACTA121750.031.2476583
ACCTCTA157050.030.2048117
GTTACCT158750.029.88003214
TAGTTGT164650.029.8762729
TTACTAC128950.029.6362274
TGTTACT130950.029.1573982
CCTACAC130450.028.93041611