FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n02_ech_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n02_ech_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25151117
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382850.15221987953855093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA115950.028.2631388
ACTACCC119450.027.6650416
TACTCTA124550.026.8344784
CTAGTTG122050.026.624458
ACTCTAG130550.026.1582975
TCTAGTT142850.024.07587
CCTACAC136200.023.98680311
CTACCCT139200.023.7654027
CTCTAGT142700.023.761926
TACTACC140150.023.677355
GTACTCT146750.023.009783
CTGTTAC147350.022.9410481
TGTACTC150150.022.5575712
ACCTCTA138850.022.48597117
TTGTACT151550.021.8955781
GGACACC132500.021.7434729
CTAATGC145550.021.26228521
CTACACT155500.021.03205712
GTTACCT149700.020.7413314
GACACCT139300.020.70694430
Produced by FastQC (version 0.11.2)