FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37209808
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT506510.13612271259233588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA80500.022.7019638
CTAGTTG108100.021.7860478
ACTACCC87550.020.8740446
GGACACC112900.018.96926529
ACTCTAG138850.017.8304145
CCTACAC120550.017.79137411
TACTCTA132600.017.6566814
ACCTCTA127700.017.5240217
CTAATGC129400.017.37385421
GACACCT128050.016.83372330
TCTAGTT149800.016.7802547
CTCTAGT147550.016.4754336
ATGCTGG141600.015.73175624
CTACCCT115850.015.6854677
GTTACCT145450.015.67001514
TACTACC117650.015.4747925
GCTGGAC142800.015.35832526
GTATGCC55200.015.320456547
TATGCCG53200.015.1828248
CGTATGC52350.015.16447146
Produced by FastQC (version 0.11.2)