FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30258128
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA60450.019.4503088
ACTACCC65200.018.1918896
CTAGTTG76800.017.958388
GCGGGCT18550.017.6657981
CCTACAC86700.015.74884711
TACTCTA95100.015.0826494
GGACACC80600.015.0179329
ACCTCTA87850.014.79678217
CCGGGGT19400.014.7580821
ACTCTAG105300.014.6367015
TCTAGTT106900.014.3209157
GCCGAGT53700.014.0677111
GACACCT90450.013.72652630
CTAATGC96500.013.3989121
TACTACC88100.013.2674635
CTCTAGT113400.013.256856
TAGCCTA85450.012.96451442
GTCCGCT17050.012.9481941
TATGCCG41200.012.81917948
ATGCTGG104350.012.78831924
Produced by FastQC (version 0.11.2)