FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685097
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT183040.13375133548560159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA35050.025.9713178
ACTACCC37650.024.6355466
CTAGTTG41200.022.8477528
ACTCTAG50450.020.7772145
TACTCTA48850.019.9049474
GGACACC44450.019.70615629
CCTACAC49950.019.32889211
CGTACGT20000.019.31986834
ACCTCTA48150.019.2636617
CTACCCT47950.019.0560177
TACTACC48500.018.8399185
TATGCCG20650.018.55416548
TCTAGTT57650.018.3019357
CTCTAGT57500.017.9898826
CTAATGC50750.017.93697221
CGTATGC21500.017.81945446
GTATGCC22400.017.72100347
CTACACT52600.017.30629712
GTTACCT54100.017.27267514
GTACGTA21950.017.1326335
Produced by FastQC (version 0.11.2)