FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21140229
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA77400.024.5011628
ACTACCC82300.023.4615786
CTAGTTG75400.022.9103288
CCTACAC94300.020.91468411
TACTCTA85900.020.9127184
ACTCTAG90850.020.4944765
CTACCCT95800.020.0471467
TCTAGTT95300.019.8628837
TACTACC98550.019.4529825
GGACACC83050.019.30875429
ACCTCTA87500.019.26915417
CTCTAGT99400.018.7316676
CTACACT106650.018.3310412
CTAATGC92800.018.02003321
GACACCT89800.017.93519830
CTGTTAC109500.017.8299061
GTTACCT97050.017.3376514
GTACTCT105000.017.2072333
TACACTC113550.017.09579313
ATGCTGG100750.016.7702124
Produced by FastQC (version 0.11.2)