FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7051274
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA140090.19867331775789737No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT132720.1882213058236001No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC119760.1698416484737368No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT79760.11311431097415872No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT78580.11144085451792116No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA72410.10269066270861124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG65850.033.4050755
TACTCTA41950.030.8207934
CTAGTTG44250.028.595888
CTCTAGT45100.028.439186
TCTAGTT46250.027.8061987
CTAGCAG35600.027.1180678
GCTGACT35600.026.8389341
GTTACCT46850.026.56829514
GTACTCT48400.026.1434063
ACCTCTA47650.025.97604217
CTAATGC47050.025.79489121
TAGTTGT50100.025.6014849
TGTACTC49950.025.470922
CTGACTC37600.025.21732
TTGTACT50700.024.8324971
GACTCTA39500.024.7020994
CCTCTAA50850.024.40916818
TACCTCT52900.024.11552616
GGACACC48150.023.77818529
GACACCT47800.023.4484430
Produced by FastQC (version 0.11.2)