FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20424684
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT559500.27393324665390173No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA335290.1641592104925589No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG228240.11174713890310371No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC221790.10858919530897027No Hit
GCCAAATGTTTGAAGGAATTGGTACCTTACTTGTACAGCTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCG216190.10584741482414121No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT212330.1039575447042412No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG208560.10211173891356165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA125150.033.171014
ACTCTAG134400.031.2728485
CTCTAGT135300.031.0143136
CTAGTTG136150.030.4916118
TCTAGTT142050.029.7588737
GTACTCT143650.028.9951533
TGTACTC146000.028.387932
TTGTACT148250.027.8063241
TAGTTGT154850.027.0545839
GTTACCT151300.026.98269714
ACCTCTA153150.026.74622217
CTAATGC155550.026.02309421
GACACCT154550.025.21596330
GGACACC155400.025.12107329
CCTCTAA165750.024.71296518
TTACCTC165750.024.67172215
TACCTCT166800.024.51617616
GCTGGAC164150.024.0946526
TGTTACC172300.023.9143413
TGGACAC164150.023.88563728
Produced by FastQC (version 0.11.2)