FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8561382
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT227220.26540107660188506No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA136370.1592850313185418No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC91960.10741256493402584No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG86900.10150230418406747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA48900.027.9223754
CTCTAGT54600.025.1967056
ACTCTAG54550.025.1564435
GTACTCT54900.024.9338443
CTAGTTG55000.024.574918
TCTAGTT57350.024.1689977
TGTACTC58900.023.0653272
ACTACCC58700.022.4969756
TAGTTGT61950.022.0405489
TTGTACT62000.021.8100031
ACCTCTA61500.021.8096217
GTTACCT61050.021.80132314
CCTACAC60050.021.70501111
CTGTTAC63800.021.302811
CTAATGC61900.021.27801521
TACCCTA61850.021.2399678
CTACCCT62200.021.1203257
GGACACC62050.020.61907229
CCTCTAA65100.020.60319718
GACACCT61350.020.40655330
Produced by FastQC (version 0.11.2)