FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12595966
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA139850.11102760995067786No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT138170.10969384960232506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG86400.026.8963435
TACTCTA62550.023.8121364
CTAGTTG61850.023.8037628
GGACACC64250.021.52214629
TCTAGTT72700.020.962447
GACACCT68250.020.56523330
CTCTAGT75350.020.2250486
GTACTCT74100.020.1936663
GTTACCT73100.020.187414
ACCTCTA73000.020.0258117
GCTGACT43800.020.0034541
TGTACTC76000.019.5531922
CTAATGC73650.019.52168521
TACCCTA58100.019.2869998
ACTACCC59800.019.0843496
TTGTACT78100.018.8591561
GGGCCCC8100.018.7294549
TACCTCT79700.018.2992216
CTACCCT61750.018.0909797
ATGCTGG79750.017.9002124
Produced by FastQC (version 0.11.2)