FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13268850
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT190220.1433583166589418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG54300.024.066288
TACTCTA59650.022.2543534
ACTCTAG63050.021.7105165
TCTAGTT64650.021.4401157
CTCTAGT66350.020.6310256
GGACACC59050.020.08963229
ACCTCTA65150.019.79331817
GTACTCT69700.019.292933
TGTACTC70450.018.9902972
GACACCT62750.018.9620230
CTAATGC67050.018.82090221
GTTACCT69350.018.64444714
GCTGGAC66050.018.06304226
TACCTCT72650.017.93986716
ATGCTGG70850.017.8611324
TTGTACT72450.017.8058721
TACCCTA49700.017.6216648
TTACCTC75550.017.20567115
TGGACAC72450.016.65872828
CCTCTAA78650.016.48354118
Produced by FastQC (version 0.11.2)