FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11648389
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT146860.1260775202476497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTG53650.025.770658
TACTCTA56000.024.5656454
ACTCTAG59900.023.4842475
TCTAGTT63250.022.7858187
CTCTAGT62050.022.2819586
GTACTCT63650.022.2089883
TGTACTC63600.022.1185932
ACCTCTA61200.022.08372717
GGACACC58000.021.9389729
TTGTACT65850.020.8999391
GACACCT61450.020.70795830
GCTGGAC63000.020.68845726
CTAATGC65500.020.5810521
GTTACCT65900.020.45665614
ATGCTGG68350.019.42189424
TACCTCT71000.018.9870716
TACCCTA56200.018.9571468
CCTACAC60650.018.87398511
ACTACCC57400.018.8010986
CCTCTAA73250.018.68602218
Produced by FastQC (version 0.11.2)