FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363455
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA189090.256795213659892No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC132570.1800377675968686No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT127210.17275857596739574No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT104310.14165904456535688No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT85620.11627693793198982No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA82850.11251511688466895No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA80260.10899774630251696No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCG78310.10634953292985427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG69050.032.4597555
TACTCTA40800.029.664674
CTAGTTG43250.028.3037328
CTCTAGT45400.026.8110856
TCTAGTT47300.026.754367
GCTGACT40450.026.0989271
GTACTCT47300.026.0258883
GTTACCT46500.025.73261314
ACCTCTA47300.025.29635817
ACTACCC45900.025.2419056
CCTACAC48750.024.9695311
TAGTTGT50700.024.8248359
CTAATGC47500.024.68186221
GACTCTA43150.024.4530624
CTACCCT47100.024.2324167
TGTACTC50350.024.1765542
CTAGCAG44050.024.032228
TACCCTA48100.024.0160278
CTGACTC44250.024.0025062
GACACCT46350.023.81168730
Produced by FastQC (version 0.11.2)