FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5568672
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT143830.2582842013320231No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA82330.1478449440010114No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG58390.10485444285459802No Hit
GCCAAATGTTTGAAGGAATTGGTACCTTACTTGTACAGCTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCG57110.10255586969388752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA21600.025.3821134
CTAGTTG24600.023.126918
TACCCTA24250.022.8919568
ACTACCC24900.022.7106136
CTCTAGT24750.022.5696166
CCTACAC26650.022.51194611
ACTCTAG25000.021.792225
TCTAGTT26800.021.7432567
CGCGGGG3200.021.5599481
CTACCCT27600.020.61337
TAGTTGT29700.019.7360959
GTACTCT28300.019.6164263
ACCTCTA27500.019.5592817
GTTACCT27350.019.28835114
CTACACT29250.019.0963412
TGTACTC29900.018.6828782
CTGTTAC31300.018.1847481
CTAATGC29300.018.1228221
GGATAAC72200.017.9646321
TTGTACT30400.017.9287991
Produced by FastQC (version 0.11.2)