FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9555312
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT216670.22675345399501343No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA128530.13451156801578013No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG97550.1020898114054256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG68150.027.321455
TACTCTA67950.027.3002364
CTCTAGT69500.026.9397266
TCTAGTT72900.026.1568137
TTGTACT70400.026.0188831
CTAGTTG72300.025.8015738
GTACTCT74800.024.8001463
TGTACTC75500.024.7077372
CCTACAC68750.023.27205711
ACTACCC67200.022.8333116
TAGTTGT82150.022.5819769
GACACCT77300.022.5312630
ACCTCTA81000.022.4332617
GTTACCT80200.022.35643614
CGCGGGG6350.022.272911
GGACACC78800.022.14416129
TACCCTA69150.021.8408328
CTAATGC81900.021.72386621
CCTCTAA83050.021.67204518
CTACCCT70650.021.5723447
Produced by FastQC (version 0.11.2)