FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12536273
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG68550.022.6352625
GTGCGCA47050.018.48516868
CTAGTTG47200.018.4829438
TACCCTA42350.018.319848
TACTCTA50400.017.9246714
GGTGCGC48850.017.6647867
CCTACAC53800.017.30523711
GCTGACT38800.017.2490541
ACTACCC46500.016.9811256
TGCGCAT52300.016.62991569
TCTAGTT57050.016.6213467
CTACCCT47850.016.3581077
ACCTCTA53750.016.29423517
GGACACC50750.016.24121729
GTACTCT59150.015.7393223
CTCTAGT60600.015.5905656
GACACCT53600.015.5070730
CTAATGC55750.015.21489421
CTAGCAG45850.015.1916398
AGGTGCG58050.015.04459866
Produced by FastQC (version 0.11.2)