FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11083199
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT113990.10284936686601044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA41000.025.229568
ACTACCC42000.024.7110616
CTACCCT46000.022.4120567
CTAGTTG47350.022.2101828
TACTCTA50250.021.4773434
CCTACAC54150.021.01336511
TCTAGTT54900.020.4117077
ACTCTAG54500.020.055575
GGACACC49300.019.79595629
GACACCT52150.018.9788730
CTCTAGT57800.018.9702686
ACCTCTA54550.018.83579617
GTTACCT56200.018.59021814
CTAATGC56250.018.20540421
CTGTTAC58300.018.1060241
TACTACC57150.018.0396145
TGTACTC60350.017.65492
GTACTCT60600.017.6384073
TTGTACT61300.017.1073721
CTACACT61350.017.0859512
Produced by FastQC (version 0.11.2)